Any software that could detect recombination in bacterial whole genome data? How far could we interpret this data?

When do we normally look at recombination in a large dataset of bacterial genomes- is this particularly important for medical pathogen? How do we normally interpret this?

08 September 2016 4,551 3 View

What are available bioinformatics tools for bacterial whole genome functional assignment/analysis (gene ontology)?

Any software pipelines that are more user-friendly for biologists to assign GO terms for bacterial whole genome data?

08 September 2016 3,187 1 View

What could be the yellow pellets seen after phenol-chloroform genomic DNA extraction?

I have done nanodrop on the samples, some were detected to be phenol contamination as seen in the yellow pellets but results were not consistent at all. I suppose yellow pellets should be protein...

01 February 2016 1,827 3 View

How do I confirm/visualise the sporulation of bacteria?

I am interested in Clostridial spore formers and wondering what are the methods to confirm the bacterial sporulation besides using microscopy?

01 February 2016 8,463 3 View

Does anyone know how to identify antimicrobial peptides secreted by bacteria?

11 December 2015 4,403 6 View

Does anyone know how to identify unknown components of gases produced by bacteria?

Does anyone know how to identify unknown components of gases produced by bacteria?

11 December 2015 7,564 0 View

Has anyone worked on IEC6 with C. perfringens (cell culture experiement)?

As IEC6 will die in anaerobic environment, C. perfringens will form spores in aerobic environment, will heat-killing method suitable in this case?

09 October 2015 6,828 1 View

How do I heat kill Clostridium spp (including C. perfringens)?

Any recommended paper or protocols? Autoclaving seems like not a good way as it might detroy the cell walls and stuff-I need intact bacterial components.

09 October 2015 5,150 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View