Does anyone know how to identify unknown components of gases produced by bacteria?

12 December 2015 0 8K Report

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Any software that could detect recombination in bacterial whole genome data? How far could we interpret this data?

When do we normally look at recombination in a large dataset of bacterial genomes- is this particularly important for medical pathogen? How do we normally interpret this?

08 September 2016 4,716 3 View

What are available bioinformatics tools for bacterial whole genome functional assignment/analysis (gene ontology)?

Any software pipelines that are more user-friendly for biologists to assign GO terms for bacterial whole genome data?

08 September 2016 3,300 1 View

Does evoglow plasmid work well with in vivo real-time imaging?

I am wondering if evoglow plasmid work well with animal whole body imaging as some say cyan-green fluorescent proteins do not work well in animals as they penetrate poorly. Any recommendations for...

03 April 2016 7,746 0 View

What could be the yellow pellets seen after phenol-chloroform genomic DNA extraction?

I have done nanodrop on the samples, some were detected to be phenol contamination as seen in the yellow pellets but results were not consistent at all. I suppose yellow pellets should be protein...

01 February 2016 1,957 3 View

How do I confirm/visualise the sporulation of bacteria?

I am interested in Clostridial spore formers and wondering what are the methods to confirm the bacterial sporulation besides using microscopy?

01 February 2016 8,742 3 View

Does anyone know how to identify antimicrobial peptides secreted by bacteria?

I have aliquoted soluble media of bacteria. Just want to know what is the best method to analyse the sample - might contain small/large antimicrobial peptides?

11 December 2015 4,532 6 View

Has anyone worked on IEC6 with C. perfringens (cell culture experiement)?

As IEC6 will die in anaerobic environment, C. perfringens will form spores in aerobic environment, will heat-killing method suitable in this case?

09 October 2015 6,944 1 View

How do I heat kill Clostridium spp (including C. perfringens)?

Any recommended paper or protocols? Autoclaving seems like not a good way as it might detroy the cell walls and stuff-I need intact bacterial components.

09 October 2015 5,279 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Who of all of you will help TPEOM to re-originate the Earths atmosphere to it original 5 atmospheric elements/gases?

Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...

06 August 2024 8,716 0 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View