What could be a suitable acid for maintaining cell culture pH?

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Alessandro Chiadò

Hi Jirka, what kind of culture you want to control the pH?

Jirka Holler

hybridoma cells - continues suspense cultivation.

Ok, I was thinking that hybridoma cell lines increase the pH during culture, but I'm not an expert of this kind of cells. Anyway, maybe this can help you:

http://link.springer.com/article/10.1007%2FBF01875556

Yes, they do increase it with time. But if we need to lower it?

As I said I'm not an expert, but I would try with a very diluted acid solution. Maybe some gly-HCl that you can filter sterilize. You should try.