Where can I get mAb secreting hybridoma clone LT56?

More Jirka Holler's questions See All

Where can one obtain mAb producing cells?

Can someone advice where can one get (buy) mAb producing cell lines for commercial use (for mAb production and sale)?

02 March 2016 6,183 1 View

Where can one get Spirulina cells for comercial use?

Can someone advice where could I get Spirulina and Chlorella cells for commercial use?

02 March 2016 5,624 4 View

What could be a suitable acid for maintaining cell culture pH?

Can someone suggest good acid for lowering pH in cell culture? Ideally an autoclavable one.

11 December 2015 8,633 5 View

PKa of oxygen?

Can one determine pKa of oxygen - O2? Or how does dissolving of oxygen in pure water affect pH?

10 November 2015 4,492 8 View

How is it with oxygen concentration?

If somewhere is such a statement "In the body, oxygen concentrationsrange from 1 to 12%", what is the oxygen concentration in mg/ml? How can one calculate this value?

10 November 2015 4,911 0 View

What are pH changes as air is dissolved in fermentation medium?

How so that when air is introduced in culture medium than pH rises and when pure oxygen is used than it does not. There is bicarbonate in medium presented and hybridoma cells cultivated.

10 November 2015 1,888 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Sunil K Joshi

ABCAM / Genetex / Generon / Mybiosource have this clone.

Jirka Holler

I do not want to get antibodies, but I want to get cells. Is there any good source?

Zirka LT56 clone may be sold to various companies. In this case find the original paper describing this clone and contact them.

It is like looking for a needle in a haystack:) I assume there is no easier way, is it?