Why people write or get Doctor of Philosophy degree even he is specialised in Basic Science or Applied Science, Commerce or Engineering?

Philosophy is the mother of all subjects... Is it a justified statement?

04 May 2019 5,636 4 View

What are the recreations for a researchers who spend days and nights in studying literature and doing experiments?

Researchers has a different mind set

04 May 2019 2,108 3 View

Will pH of seawater decrease if DIC increases?

Acidification

02 March 2019 8,604 1 View

Can meditation improve our concentration?

What is the scientific reason behind this?

31 December 2018 2,253 13 View

Are always Open Access publications not up to the mark?

OPEN ACCESS ARE BENEFICIAL AS THE ARTICLES ARE REACHABLE TO ALL WITHOUT COSTING

07 August 2018 8,829 3 View

How you are thinking to celebrate the World Environment day on 5th of June?

Just a speech, few claps and plantation -- every thing is over?

04 May 2016 2,584 22 View

Are we selling our future for dirty short term profitable industry?

We may soon follow the foot steps of dinosaur.. and the poorest of the poor will be the worst sufferer.

04 May 2016 6,759 22 View

How global warming has affected our economy and GDP?

GLOBAL WARMING HAS INCREASED THE SALE OF SUN SCREEN LOTION, COOLING POWDER, AC etc.

03 April 2016 7,681 30 View

Is it really necessary to change our attitude with time?

Change is inevitable, but sometimes remain stagnant...

03 April 2016 8,610 35 View

What is self determination? Is it a confidence to move freely scratching all bonds?

The symptom of free will is self-determination. Non-living bodies obey mechanical laws of nature and act according to external forces. But the living organisms utilize physical laws in carrying...

03 April 2016 513 16 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View