Hello all.
I was wondering if anyone has any experience with adding ampicillin to the medium in which a fungi is stored and what concentration is best to use?
I know normally there shouldn't be any of the antibiotics and if, the one usually added is CHL but that's the decision that was made. So does anyone have a suggestion on what concentration is suitable to grow the fungi and inhibit any possible contaminants?
Thank you for the help in advance have a lovely weekend.
Best regards, I.
Ampicillin is not the most stable antibiotic in wet environments. Thus, it will lose effect soon. It will also not attack non-dividing basteria. It is also not recommendable to use any antibiotic at all for storage of fungal isolates. If you go for single spore isolates, you will not need it.
Hi Ivana Bokan
I agree with Dr. Johannes Wostemeyer and Dr. Phil Geis here. What I usually do when it is needed to store fungal cultures for long-term storage is first I obtain the pure culture then grow it in a broth and store it in cryovials with a cryoprotectant at -80 C.We do not use antibiotics when storing pure cultures.
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