What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

03 December 2020 208 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

24 November 2020 6,612 7 View

Do you have experience visualising multiplex amplification curves in LightCycler 480 Sw??

We are using Cobas z480 for multiplex RT-PCR using 3 taqman probes FAM/VIX/CY5. Once the PCR finish we want to see in the amplification curve the three results for each sample at the same time....

28 October 2020 8,372 1 View

TEACHING ESL in the lockdown - participants needed?

Dear English lecturers (teachers, instructors), I am conducting research into ESL teaching to increase our understanding of how teachers have experienced special teaching conditions during the...

14 October 2020 7,223 6 View

Why does mitotracker red stains the nucleus?

I'm working with NT2 cells and I use mitrotracker red (400 nM) to stain the mitochondria. I incubate it with cell medium for 30 min, fix with 4% PFA, permeabilize with PBST (0.2%-0.5% Triton...

11 August 2020 8,076 3 View

What psychometric properties should be reported for a translated version of a questionnaire?

Dear collegues, I performed a cross-cultural study using two questionnaires (66 and 12 items). The original version of these questionnaires was used in the first country, and these tools were...

27 April 2020 4,097 9 View

USRP N210_ RF1 and RF2 dont work ?

Hi, I am working on USRP N210 and Matlab 2016R. I want a transfer a Sinusoidal wave from one port of Antenna and want to receive same wave from second Antenna at one USRP N210 (unfortinaly I...

28 January 2020 5,568 3 View

Problem in modulating miRNA expression (both over-expression and knock-down) in mouse cell lines?

Hi everybody, we are working with let-7 family and we would like to perform knock-down of the let-7 using Lin28 expression vector. In another experiment we would like to over-express let-7g from a...

20 September 2017 9,420 2 View

Can anyone help with a PCR insertion/deletion genotyping problem?

09 July 2014 4,184 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How to find fast publication Scopus Indexed (Q2) journals in Computer Science and Engineering?

Dear Researchers, I would like to find some (Q2) Scopus-Indexed journals with fast publication in the field of Computer Science and Engineering major?

28 February 2021 3,285 2 View