What is the best protocol for chemical/electro transformation for anaerobic bacteria?

12 December 2022 1 10K Report

Which protocols can be used for transformation of anaerobic bacteria from Azoarcus genus? So far I tried chemical transformation with CaCl2 buffer and electrotransformation with MOPS, but they didn't work. Any suggestions, tips & tricks?

Mahendra Raut

WOuld this be helpful

Please check this paper "Agr Quorum Sensing infuences the Wood‑Ljungdahl pathway in Clostridium autoethanogenum" 2021

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