What company would you recommend to buy ELISA kits from?

More Reza Sanaei's questions See All

How can I remove Tween 20 from rhProtein solution by dialysis?

The supplier states that in addition to the recombinant protein, the solution is formulated in 240 mg α,α‑trehalose dihydrate, 23.2 mg sodium phosphate (monobasic, monohydrate), 4.8 mg sodium...

08 September 2016 3,505 7 View

Help me with replacing Deuterium lamp in a Spectrophotometer?

Our UV/vis Spec. measurements are no longer repeatable, after a minute or two the absorbance of the same solution in the same cuvette changes significantly. Sometimes the absorbance is zero or...

08 September 2016 6,901 6 View

How to measure gel fraction of a peace of polymer by soxhlet extraction?

I am given a task to extract and determine the gel fraction of a peace of PVA polymer. My concern is the measurement of gel residue on thimble. How can I quantify this quantity?

06 July 2016 8,135 4 View

What does it mean to reach from 10X PBS to 1X PBS by dilution?

What follows is part of protocol for ECM digestion. " The pepsin was first solubilized in 0.1 M HCl and added to the adipose matrix at a ratio of 1 mg pepsin for every 10 mg lyophilized ECM. The...

03 April 2016 2,033 5 View

Which protein content assay can work under microgram values?

Among all different protein content assays (BCA, Lowry, Bradford, etc) which one would you recommend for under microgram amounts of protein mixtures?

03 April 2016 7,729 5 View

How can I make sure that silica particles left over in Lab. shelf are in good quality after some years?

After some years the silica particles might have received moisture or some other stuff fumes. I am thinking how can I make sure the particles are in the same quality as once made? Or if there is...

03 April 2016 809 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Jordan R. Yaron

It will entirely depend on the cytokine that you want to measure. For the cytokines that I am interested in, I usually buy my kits from R&D Systems.

Mike Zhang

Novatein Biosciences is my choice as they give discount if you say you have funding crunch!

Kallyne Oliveira Mimura

I usually buy my ELISA kits from BD or R&D Systems.

WA Gadalla

I always use the kits from

R&D Systems

IBL International GmbH - Hamburg, Germany

Dan Peng

I recommend Creative Diagnostics company:

https://www.creative-diagnostics.com/