WA Gadalla

9 Questions 5 Answers 0 Followers

Questions related from WA Gadalla

Could you please help me to know if this digested DNA is good enough to detect SNP or it must be done again?

Hello everyone I hope all is well I want to know what is wrong with my experiment as DNA was extracted and after digestion by restriction enzyme it gives a very faint bands and also I want to...

06 June 2019 4,955 1 View

How can I prepare Giemsa stain?

Dear colleagues, I want to know how can I prepare the Giemsa stain as the last time I prepared it , it was light, not stable, and be faint after 2 days

09 September 2017 9,848 3 View

How can i use medcalc software to calculate sensitivity , specificity , ppv, npv of Combinations of 2 markers ?

Hello everyone, I hope all is well, I want to calculate the sensitivity of combined marker together to know if combination between them can be used to better predict the disease. here is a pic of...

05 May 2017 7,156 1 View

Is there any reason that make protein show difference between groups but no difference have been showed by real time PCR?

Hello everyone, when I studied certain protein in patients who had different stage of liver disease (HCV, liver cirrhosis, HCC) and control group, I surprised that ELISA showed difference in the...

03 March 2017 7,654 3 View

What is the reason that cause RNA dose not appear on the gel?

I am trying to extract RNA from whole blood , first I am collecting the blood on EDTA tube put it in ice box until I reach to my lab this take about 30-45 mins then put it in -20c until second day...

11 November 2016 4,316 6 View

What is the protocol of using DEPC in RNA extraction ?

I want to extract RNA by Direct-Zol RNA Miniprep kit from sigma but the kit didn't provide RNase free tubes and one of my colleagues advised me to use diluted DEPC to sterilize normal Eppendorf...

10 October 2016 9,376 7 View

I want to ask about the validity of my samples?

Good evening, everyone. I hope all is well I want to ask about the validity of my samples, since they were Reserved as a whole blood since 2012 on EDTA tube (CBC tube with purple cap) at -20C and...

09 September 2016 8,054 7 View

Why blank value is higher than sample value in ELISA test? and Is there any way to avoid negative values after substract sample value from blank?

blank 0.208 values of samples ranges from (0.165-2.2080)a lot of sample values below blank value

05 May 2016 7,566 16 View

How can I detect fgf21 rs838133 SNP by conventional PCR?

Hi everyone I want to detect FGF21 SNP rs838133 by conventional PCR method but all data published used rt-PCR method. Thus, I want to ask is there are any to do it by conventional PCR & how...

01 January 1970 6,936 1 View