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Questions related from Reza Sanaei
The supplier states that in addition to the recombinant protein, the solution is formulated in 240 mg α,α‑trehalose dihydrate, 23.2 mg sodium phosphate (monobasic, monohydrate), 4.8 mg sodium...
09 September 2016 3,457 7 View
Our UV/vis Spec. measurements are no longer repeatable, after a minute or two the absorbance of the same solution in the same cuvette changes significantly. Sometimes the absorbance is zero or...
09 September 2016 6,855 6 View
I am given a task to extract and determine the gel fraction of a peace of PVA polymer. My concern is the measurement of gel residue on thimble. How can I quantify this quantity?
07 July 2016 8,067 4 View
What follows is part of protocol for ECM digestion. " The pepsin was first solubilized in 0.1 M HCl and added to the adipose matrix at a ratio of 1 mg pepsin for every 10 mg lyophilized ECM. The...
04 April 2016 1,942 5 View
Among all different protein content assays (BCA, Lowry, Bradford, etc) which one would you recommend for under microgram amounts of protein mixtures?
04 April 2016 7,699 5 View
After some years the silica particles might have received moisture or some other stuff fumes. I am thinking how can I make sure the particles are in the same quality as once made? Or if there is...
04 April 2016 765 2 View
I need to quantify the amount of some cytokines in serum and culture supernatant. Different kits have different sensitivity and range of detection, what brand or brands would you recommend?
03 March 2016 3,371 5 View
