For a while now, my cell culture has consistently been contaminated by Yeast. I had observed all aseptic procedures l could observe, yet the contamination kept occurring. Just do not know what could possibly be responsible for this since only my culture is being contaminated among 5 of us working with cell cultures. Could this be an indication l need to go for a medical check up or could it just be due to environmental contamination. I need urgent help.
Use antibiotic with fungicide.
Gibco® Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Amphotericin B prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast.
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