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Questions related from ALEX CHUN CHEUNG
I would like to set up a laboratory with AAALAC accreditation and I have a few questions on it. 1. About the exportation of animal, in the program description, it mentioned that "Describe how...
30 August 2020 7,306 0 View
I can't get the DNA sequence of any genes coding for L-Alanine: 4,5-dioxovalerate transaminase. Another strange thing is that I can't also get the protein sequence of this enzyme. KEGG, NCBI and...
25 June 2017 7,143 2 View
Currently, there are two major blood group classification systems. There are ABO blood group and Rh blood group. However, these two types are based on RBC surface antigen. I have two...
07 May 2017 1,501 5 View
I have cloned the wild type sequence of gyrB or rpsL gene of Mtb from strain H37Ra into PVV16 vector and transform the plasmid to mc2 155 (Segmentis strain). However, for overexpression of gyrB,...
17 January 2017 2,191 2 View
I would like do some research on melanogenesis in hair follicle melanocytes. However, most of the melanoma cell line are derived from skin. I found an article describing isolation of melanocytes...
19 November 2015 10,072 3 View
can the medium and all its components for culturing E14 mESCs be filtered without any undesirable effect? STANDARD ES CELL MEDIA (600 ml) (F. Mariani 03/12/2003):ES CELL PROTOCOLS (for ES Cells...
18 March 2015 6,331 2 View
What is the technique and skills required to culture E14 (mESC) to keep it passing without differentiation?
11 March 2015 1,603 2 View
Currently, I am trying to do karyotyping on HeLa cells. The protocol is as followed. HeLa cell karyotyping protocols1. Culture the HeLa cells in a 6 well-plate with 80% confluence.2. Discard the...
27 February 2015 7,340 6 View
