Joshua Adekunle Babalola

9 Questions 5 Answers 0 Followers

Questions related from Joshua Adekunle Babalola

In presenting data from qPCR, which is appropriate relative fold change or ddCt?

In analyzing data from my qPCR experiments, analysis on GraphPad is done using the ddCt, in presenting the analysis in graph, which is the most appropriate, the relative fold change or the ddCt?

06 January 2023 9,044 0 View

Could behavioural testing give different results in knockout and transgenic mice?

I did Morris Water maze and Contextual fear conditioning experiments on Alzheimer's disease transgenic mice in which type 2 diabetes phenotype was induced using low dose streptozocin injection and...

27 August 2022 6,450 0 View

Does Type 2 diabetes reduces the threshold for neurodegeneration in Alzheimer disease (AD) from clinical perspective?

Hi all, l would appreciate clarification on whether type 2 diabetes reduces the threshold for neurodegeneration in Alzheimer disease (AD) from clinical perspective as it does when it is induced in...

19 June 2022 7,183 2 View

What is the appropriate fasting period prior to investigating intraperitoneal Glucose or Insulin sensitivity test?

Experts in the field of metabolism, what is the appropriate fasting period prior to investigating intraperitoneal Glucose or Insulin sensitivity test? This publication:...

08 February 2022 7,124 1 View

Can anybody help with suggestions on antibodies that can detect GLUT1; IDE and NEP in porcine cells?

I work with porcine cells and most antibodies are not validated in this specie. I would appreciate suggestions on antibodies to detect GLUT 1; Insulin degrading enzyme and Neprilysin in porcine...

26 February 2021 5,993 1 View

Please l need recommendation on antibodies that can detect Chronic Wasting Diseases Prion Protein in cell lysate or brain tissue homogenate?

6H4 monoclonal antibody known to be effective in capturing cervids Prion Proteins has been discontinued by Prionics. I need suggestions on antibodies either monoclonal or polyclonal that could be...

29 December 2016 3,073 2 View

My transfected cells do grow at sporadic rate a week after subjecting them to antibiotic selection treatment , what could likely cause this?

I am trying to establish a stable cell line expressing a gene of my choice using a prion protein knock out cell line, Lipofectamine and Zeocin as selection antibiotics. Not ruling out the fact...

26 December 2016 6,158 6 View

What could be the likely causes of false positive or false negative results during PCR reaction?

I did tail biopsy to genotype a particular gene. The result came out positive for my desired gene but two days after using same lysed genomic DNA, it came out negative. In my confused state, l...

02 December 2016 5,217 6 View

Please help with expert views based from experience on establishing cell lines expressing prion protein gene from any of the cervids- elk, deer ?

I need help on the most appropriate transfection approach and conditions to optimize cell transfection when establishing stable cell lines expressing any of the cervids' prion protein genes and...

13 July 2016 2,186 0 View