Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Is it possible to full-couple the interFoam solver with the chtMultiRegionFoam solver?

We need to calculate multiphase stream of the one substance in the air atmosphere (this is why we using interFoam), but also we need to get heat transfer data between the substance, solid obstacle...

11 January 2021 872 2 View

Does anyone have experience with Land use/land cover data extraction from Second military survey maps?

I am trying to create polygon shapefile with land use from Second military survey using Arcmap. However, I really have hard time with reading the map, more correctly with boundaries between...

07 November 2020 6,018 3 View

How do I remove Bacillus sp. spores from Kraft Paper machine?

In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...

03 November 2020 1,726 10 View

What volume of a cell lysis buffer does one use for cells?

I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...

03 November 2020 6,330 3 View

Best protocol to perform Western Blot from mice brain?

I am trying to look at the expression of c-fos nuclear protein in mice brain using WB technique. Can anyone suggest a protocol to extract the proteins? Better harsh homogenate before sonication?...

27 October 2020 5,272 2 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

25 October 2020 8,600 1 View

What do you think now of virtual teaching compared to face-to-face teaching of finished transmission of knowledge?

21 September 2020 4,178 10 View

Has anynone successfully used NEB's TtAgo (Thermus Thermophilus Argonaute) for any type of application?

I have been trying to use NEB's TtAgo (https://www.neb.com/products/m0665-ttago) to linearize supercoiled plasmids, following NEB's recommendations, but I haven't had any success yet (19/19 failed...

09 September 2020 2,112 1 View

How to store W/O droplets for over 10 days using paraffin oil with Span 80?

I am currently using a PDMS chip to produce water droplets with a diameter of 200 um in paraffin oil with 3wt%. After a couple of hours, the stationary droplets stored in the chip starts to form...

18 August 2020 2,459 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View