Ivan Restrepo

4 Questions 7 Answers 0 Followers

Questions related from Ivan Restrepo

What blocking buffer should I use for detecting albumin by WB?

Hi! I want to run a western blot to detect albumin from serum sample. I usually use BSA as blocking buffer, but obviously, I can't use it for this case. What blocking buffer would you recommend...

27 October 2017 5,285 3 View

Why do I see two bands on an agarose gel (4%) after doing RT-PCR of a short, synthesized DNA oligo (approx 24 nt)?

Hello, I am assessing the expression of a miRNA in cells Hep-G2. I am using a stem-loop primer and pulsed RT to make DNA from my miRNA. I am also running agarose gels, at 4%, to visualize my PCR...

01 April 2017 5,939 4 View

I want to compare the expression levels of some miRNAs in rhabdomyosarcoma cell lines vs control cell lines. Which normal cell lines should I use?

I am planning to use tumor cell lines from alveolar Rhabdomyosarcoma.

02 April 2015 573 0 View

I am looking for a protocol to isolate apoptotic bodies from the supernatant of cultured cells. I will appreciate any information about it.

I am trying to set up the optimal conditions to isolate apoptotic bodies, in order to extract DNA. Thanks for your help

14 August 2013 421 2 View