I have noticed morphological changes in primary melanoma cell cultures after switching to a different brand of FBS, the cells are circled in blue. Some cells began to form a more round shape with black particles attached to and surrounding the cells.
After the removal of antibiotics, the black particles seemed to increase as free floating particles with no particular motion. I assumed that it was mycoplasma contamination and tested them accordingly via pcr. The results were negative. I then assumed that it was potentially apoptotic bodies from low quality FBS so I decontaminated everything and purchased new FBS. After activating a stock, the cells seemed to be much healthier but after a few passages, have begun to exhibit the same changes again.
The cell passage number is
Hello Cassandra Herbert
I would like to draw your attention to the two articles attached below. May be it will be helpful.
https://www.sciencedirect.com/science/article/abs/pii/S1045105609001304?via%3Dihub
https://www.biorxiv.org/content/10.1101/366906v1
Best Wishes.
your cells’s phase daek dots look like membrane blebs. These are transient, repeated outpouchings of plasma membrane. This excessive membrane activity is a culture artifact usually due to medium growth factor
Article Membrane blebbing is associated with Ca2+-activated hyperpol...
https://www.nature.com/articles/s41598-017-16825-
Article Charras GTA short history of blebbing. J Microsc 231:466-478
Article Dynamic blebbing: A bottleneck to human embryonic stem cell ...
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