Please suggest me some articles or sites about this topic.
PCR
restriction enzyme
Sequencing
Targeted gene inactivation in Salmonella Typhi by CRISPR/Cas9-assisted homologous recombination | Request PDF (researchgate.net)
What selectable marker genes do you suggest instead of hygromycin in the gene knockout project in rice?
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Dear Sir and Ma'am, We faced a problem in the industry that couldn't be solved despite running several tests. "When using chicken thighs for making sausages, we noticed weak tissue in certain...
11 January 2024 4,415 0 View
After designing gRNA, how should we make it? I need an article, site or instruction on this please
26 September 2023 9,258 0 View
What are the ways to confirm the edited gene through CRISPR system?
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what is the knowledge gap in tourism research?
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I have to draw a phylogeny tree using their complete sequence on the NCBI website
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I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...
07 December 2021 9,234 7 View
I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...
21 November 2021 9,368 3 View
Hi, I am looking for this book "introduction to magnetic material" by Cullity , how can I find it?
16 January 2020 229 2 View
Hi, I am looking for a solution manual of this book "introduction to magnetic material" by Cullity , how can I find it?
16 January 2020 3,695 2 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
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Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
I am looking for a CRISPR protocol for application in leishmania strains
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I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...
03 June 2024 2,877 7 View
Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...
30 May 2024 9,993 0 View