After designing gRNA, how should we make it? I need an article, site or instruction on this please
What selectable marker genes do you suggest instead of hygromycin in the gene knockout project in rice?
14 May 2024 2,680 0 View
Dear Sir and Ma'am, We faced a problem in the industry that couldn't be solved despite running several tests. "When using chicken thighs for making sausages, we noticed weak tissue in certain...
11 January 2024 4,415 0 View
Please suggest me some articles or sites about this topic.
22 September 2023 2,553 1 View
What are the ways to confirm the edited gene through CRISPR system?
22 September 2023 3,290 3 View
what is the knowledge gap in tourism research?
10 May 2023 4,024 4 View
I have to draw a phylogeny tree using their complete sequence on the NCBI website
19 April 2023 8,745 0 View
I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...
07 December 2021 9,234 7 View
I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...
21 November 2021 9,368 3 View
Hi, I am looking for this book "introduction to magnetic material" by Cullity , how can I find it?
16 January 2020 229 2 View
Hi, I am looking for a solution manual of this book "introduction to magnetic material" by Cullity , how can I find it?
16 January 2020 3,695 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View