Ido From

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Questions related from Ido From

RT step- Can I use 2 different target reverse primers instead of random hexamers? For 2 different PCR reactions on the same "first strand" RT product?

For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...

23 August 2023 1,549 1 View

What are the universal primer sequnces for H5N1 amplification by RT-PCR (for phylogenetic analysis purpose)?

Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.

28 November 2022 9,582 2 View

How do I measure RNA integrity of pure viral RNA?

In our diagnostic lab, we extract pure viral RNA (Qiagen viral RNA extraction kit). Therefore, no 28S and 18S rRNA can be traced in agarose gel for total RNA integrity assesment. However, very...

25 October 2022 6,919 7 View

Can you reproductively create an end point RT-PCR amplicon product originating at purified viral RNA sample from direct tracheal/cloaca swabs?

Hi to all, We successfully created in our lab a new RT-PCR system, designed for amplification of the S1 segment of Infectious bronchitis virus (IBV). Yet, we obtained the end point PCR product...

28 October 2021 7,898 2 View

Is there a universal qRT-PCR system (Taqman) to detect Infectious bronchitis virus (including QX variants)?

Hello, We have a qRT-PCR system (primers and probe) that does not detect the QX variant. Does anyone knows a universal set of primers and probe for universal detection of IBV? Thanks in...

03 June 2021 7,234 9 View