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For WGS, we need to obtain pellets of Avibacterium paragallinarum (Pasteurellacea-like baceria). What are the preffered centrifuge conditions for this bacterial family? Thanks
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For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...
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Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.
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In our diagnostic lab, we extract pure viral RNA (Qiagen viral RNA extraction kit). Therefore, no 28S and 18S rRNA can be traced in agarose gel for total RNA integrity assesment. However, very...
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Hi to all, We successfully created in our lab a new RT-PCR system, designed for amplification of the S1 segment of Infectious bronchitis virus (IBV). Yet, we obtained the end point PCR product...
28 October 2021 7,912 2 View
Hello, We have a qRT-PCR system (primers and probe) that does not detect the QX variant. Does anyone knows a universal set of primers and probe for universal detection of IBV? Thanks in...
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