What are the tools available to predict disulfide connectivity in proteins?

More Boopathi Subramani's questions See All

What is the role of glutamine residues outside the catalytic site in a Beta-glycosidase?

I'm working with a beta-glycosidase from a hyperthermophilic archaeon. Eventhough it is highly thermostable, its kcat values are very low. So we tried to mutate some catalytic site and non...

03 April 2015 293 7 View

How do I perform calculation of pseudo first order kinetic parameters from experimental data?

I need to calculate the pseudo first order kinetic parameters from the PL spectral data. The reactants include a fluorescent compound (A; used in excess) and different concentration of a...

31 December 2014 2,022 4 View

How to calculate activation energy (Ea) from a non-linear Arrhenius plot?

I have to calculate the activation energy (Ea) for a thermostable enzyme (wild-type) and its mutants based on its enzyme activity (ln of activity vs 1/T). The wild-type is stable at near boiling...

01 February 2014 3,548 7 View

Can N-Terminal His-tag change the properties of the expressed recombinant protein?

We expressed a B-Glycosidase enzyme in Ecoli BL21 cells with a N-Terminal His-tag. The same enzyme has been expressed in BL21 previously but without His-tag. The enzyme with His-tag showed a...

02 March 2013 1,755 18 View

Is enzyme kinetics determined from discontinuous enzyme assay reliable?

To study the kinetics of an enzyme, continuous enzyme assay was the most preferred method. But in some cases, the discontinuous assay has been used. So how reliable are the kinetic data obtained...

09 October 2012 10,047 6 View

What is the action of Sodium Carbonate as stop solution in a discontinuous enzyme assay?

Sodium Carbonate is used as a stop solution in enzyme assays. How does it act on the enzyme substrate complex to stop the reaction?

07 August 2012 7,042 9 View

Procedure for Transglycosylation reaction of a hyperthermophilic Beta-Glycosidase

Can anyone suggest a method to study the transglycosylation reaction of Beta-Glycosidase from a hyperthermophilic organism. I prefer to use TLC to analyze the transglycosylated products.

07 August 2012 3,449 1 View

Molar extinction coefficient of 2-nitrophenol and 4-nitrophenol

I am doing Beta-Glucosidase assay from a hot spring archaea. The assay conditions include Sodium citrate buffer, pH 5.0, temperature at 70C and the substrates are 2-Nitrophenylglycoside and...

06 July 2012 3,087 5 View

Free softwares for Enzyme kinetics

Can anyone recommend the free softwares available for enzyme kinetics.

06 July 2012 1,574 1 View

Application of DSC for enzyme thermostability

I am characterizing an enzyme from hyperthermophilic organism. For measuring the thermostability of the enzyme, what are the methods available? Is using Differential Scanning Calorimetry (DSC)...

05 June 2012 7,827 11 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

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How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

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09 August 2024 5,048 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

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08 August 2024 718 0 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

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06 August 2024 9,986 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

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Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

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05 August 2024 453 3 View

The Curse of Evolution and Complexity?

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05 August 2024 6,247 3 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

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05 August 2024 3,665 0 View

Jeffrey R Brender

There are several:

DIANNA: http://clavius.bc.edu/~clotelab/DiANNA/

CysState: http://bioserv.rpbs.jussieu.fr/cgi-bin/CysState

DiPro:http://download.igb.uci.edu/bridge.html

DisLocate: http://betaware.biocomp.unibo.it/dislocate

a more complete list can be found in these two reviews:

http://www.sciencedirect.com/science/article/pii/S1876107010001021

http://benthamscience.com/cbio/Samples/cbio2-1/0004CBIO.pdf

Aparna Islam

I would suggest you to use CYS-REC (http://linux1.softberry.com/berry.phtml?topic=cys_rec&group=programs&subgroup=propt) . There are others as well as mentioned in the previous answers.

Regards.