What is the role of glutamine residues outside the catalytic site in a Beta-glycosidase?

I'm working with a beta-glycosidase from a hyperthermophilic archaeon. Eventhough it is highly thermostable, its kcat values are very low. So we tried to mutate some catalytic site and non...

03 April 2015 145 7 View

How do I perform calculation of pseudo first order kinetic parameters from experimental data?

31 December 2014 1,850 4 View

How to calculate activation energy (Ea) from a non-linear Arrhenius plot?

I have to calculate the activation energy (Ea) for a thermostable enzyme (wild-type) and its mutants based on its enzyme activity (ln of activity vs 1/T). The wild-type is stable at near boiling...

01 February 2014 3,385 7 View

Can N-Terminal His-tag change the properties of the expressed recombinant protein?

We expressed a B-Glycosidase enzyme in Ecoli BL21 cells with a N-Terminal His-tag. The same enzyme has been expressed in BL21 previously but without His-tag. The enzyme with His-tag showed a...

02 March 2013 1,636 18 View

Is enzyme kinetics determined from discontinuous enzyme assay reliable?

To study the kinetics of an enzyme, continuous enzyme assay was the most preferred method. But in some cases, the discontinuous assay has been used. So how reliable are the kinetic data obtained...

09 October 2012 9,898 6 View

What is the action of Sodium Carbonate as stop solution in a discontinuous enzyme assay?

Sodium Carbonate is used as a stop solution in enzyme assays. How does it act on the enzyme substrate complex to stop the reaction?

07 August 2012 6,508 9 View

Procedure for Transglycosylation reaction of a hyperthermophilic Beta-Glycosidase

Can anyone suggest a method to study the transglycosylation reaction of Beta-Glycosidase from a hyperthermophilic organism. I prefer to use TLC to analyze the transglycosylated products.

07 August 2012 3,333 1 View

Molar extinction coefficient of 2-nitrophenol and 4-nitrophenol

I am doing Beta-Glucosidase assay from a hot spring archaea. The assay conditions include Sodium citrate buffer, pH 5.0, temperature at 70C and the substrates are 2-Nitrophenylglycoside and...

06 July 2012 2,738 5 View

Free softwares for Enzyme kinetics

Can anyone recommend the free softwares available for enzyme kinetics.

06 July 2012 1,411 1 View

Application of DSC for enzyme thermostability

I am characterizing an enzyme from hyperthermophilic organism. For measuring the thermostability of the enzyme, what are the methods available? Is using Differential Scanning Calorimetry (DSC)...

05 June 2012 7,665 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View