Boopathi Subramani

11 Questions 69 Answers 0 Followers

Questions related from Boopathi Subramani

What is the role of glutamine residues outside the catalytic site in a Beta-glycosidase?

I'm working with a beta-glycosidase from a hyperthermophilic archaeon. Eventhough it is highly thermostable, its kcat values are very low. So we tried to mutate some catalytic site and non...

04 April 2015 241 7 View

How do I perform calculation of pseudo first order kinetic parameters from experimental data?

I need to calculate the pseudo first order kinetic parameters from the PL spectral data. The reactants include a fluorescent compound (A; used in excess) and different concentration of a...

01 January 2015 1,971 4 View

How to calculate activation energy (Ea) from a non-linear Arrhenius plot?

I have to calculate the activation energy (Ea) for a thermostable enzyme (wild-type) and its mutants based on its enzyme activity (ln of activity vs 1/T). The wild-type is stable at near boiling...

02 February 2014 3,496 7 View

What are the tools available to predict disulfide connectivity in proteins?

I am looking for some tools to predict the cysteine bonding state and disulfide connectivity of a protein from its sequence.

04 April 2013 2,638 2 View

Can N-Terminal His-tag change the properties of the expressed recombinant protein?

We expressed a B-Glycosidase enzyme in Ecoli BL21 cells with a N-Terminal His-tag. The same enzyme has been expressed in BL21 previously but without His-tag. The enzyme with His-tag showed a...

03 March 2013 1,715 18 View

Is enzyme kinetics determined from discontinuous enzyme assay reliable?

To study the kinetics of an enzyme, continuous enzyme assay was the most preferred method. But in some cases, the discontinuous assay has been used. So how reliable are the kinetic data obtained...

10 October 2012 10,020 6 View

What is the action of Sodium Carbonate as stop solution in a discontinuous enzyme assay?

Sodium Carbonate is used as a stop solution in enzyme assays. How does it act on the enzyme substrate complex to stop the reaction?

08 August 2012 6,918 9 View

Procedure for Transglycosylation reaction of a hyperthermophilic Beta-Glycosidase

Can anyone suggest a method to study the transglycosylation reaction of Beta-Glycosidase from a hyperthermophilic organism. I prefer to use TLC to analyze the transglycosylated products.

08 August 2012 3,415 1 View

Free softwares for Enzyme kinetics

Can anyone recommend the free softwares available for enzyme kinetics.

07 July 2012 1,514 1 View

Molar extinction coefficient of 2-nitrophenol and 4-nitrophenol

I am doing Beta-Glucosidase assay from a hot spring archaea. The assay conditions include Sodium citrate buffer, pH 5.0, temperature at 70C and the substrates are 2-Nitrophenylglycoside and...

07 July 2012 3,032 5 View

Application of DSC for enzyme thermostability

I am characterizing an enzyme from hyperthermophilic organism. For measuring the thermostability of the enzyme, what are the methods available? Is using Differential Scanning Calorimetry (DSC)...

06 June 2012 7,778 11 View