What is the role of glutamine residues outside the catalytic site in a Beta-glycosidase?

I'm working with a beta-glycosidase from a hyperthermophilic archaeon. Eventhough it is highly thermostable, its kcat values are very low. So we tried to mutate some catalytic site and non...

03 April 2015 145 7 View

How do I perform calculation of pseudo first order kinetic parameters from experimental data?

31 December 2014 1,850 4 View

How to calculate activation energy (Ea) from a non-linear Arrhenius plot?

I have to calculate the activation energy (Ea) for a thermostable enzyme (wild-type) and its mutants based on its enzyme activity (ln of activity vs 1/T). The wild-type is stable at near boiling...

01 February 2014 3,385 7 View

What are the tools available to predict disulfide connectivity in proteins?

I am looking for some tools to predict the cysteine bonding state and disulfide connectivity of a protein from its sequence.

03 April 2013 2,566 2 View

Can N-Terminal His-tag change the properties of the expressed recombinant protein?

We expressed a B-Glycosidase enzyme in Ecoli BL21 cells with a N-Terminal His-tag. The same enzyme has been expressed in BL21 previously but without His-tag. The enzyme with His-tag showed a...

02 March 2013 1,636 18 View

Is enzyme kinetics determined from discontinuous enzyme assay reliable?

To study the kinetics of an enzyme, continuous enzyme assay was the most preferred method. But in some cases, the discontinuous assay has been used. So how reliable are the kinetic data obtained...

09 October 2012 9,898 6 View

What is the action of Sodium Carbonate as stop solution in a discontinuous enzyme assay?

Sodium Carbonate is used as a stop solution in enzyme assays. How does it act on the enzyme substrate complex to stop the reaction?

07 August 2012 6,508 9 View

Molar extinction coefficient of 2-nitrophenol and 4-nitrophenol

I am doing Beta-Glucosidase assay from a hot spring archaea. The assay conditions include Sodium citrate buffer, pH 5.0, temperature at 70C and the substrates are 2-Nitrophenylglycoside and...

06 July 2012 2,738 5 View

Free softwares for Enzyme kinetics

Can anyone recommend the free softwares available for enzyme kinetics.

06 July 2012 1,411 1 View

Application of DSC for enzyme thermostability

I am characterizing an enzyme from hyperthermophilic organism. For measuring the thermostability of the enzyme, what are the methods available? Is using Differential Scanning Calorimetry (DSC)...

05 June 2012 7,665 11 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View