Suitable framework for federated learning experiments?

As we have TFF and PySyft which are most commonly being used for testing FL algorithms, which one goes better with IoT devices?

22 September 2020 4,407 3 View

Can anyone please suggest the name of a fluorescent dye which is soluble in water?

I need a fluorescent dye which is soluble in water. However, I am yet to come across something which is suitable. I would be using the dye to see the behaviour of aqueous solutions in a porous...

14 September 2020 3,888 4 View

How to use bibliometric softwares?

Dear All, Can we use software like bibexcel. VoSviewer with the "ris" or "bib" files downloaded from the Journals page. Usually, a database from WoS or Scopus or PubMed is used, but if one does...

01 May 2020 9,612 6 View

Do we need a UDF for transient compressible simulation in Fluent?

I observed in some manuals and websites for performing transient simulation in Fluent that UDF (sinusoidal profile of pressure) has been used at the pressure outlet of the domain. Is it necessary...

31 October 2019 5,648 3 View

Does gravitational waves have energy associated with it? If it does have energy then it imply the need of quantization of gravitational energy?

According to GTR, gravity is not a force but effects of gravity is because of the shortest path which object follows. Since locally the space time is flat then one cant define energy density...

04 August 2019 7,960 15 View

How to do quantification of Hydroxypropyl-beta-cyclodextrin ?

Will UV-Vis spectroscopy method will work ?

26 May 2019 9,377 3 View

Which is the best classical force field?

I want to perform molecular dynamics simulation on some metal-oxides nanoparticles in different solvent mediums such as water, ethanol etc. Any help.

07 February 2019 5,129 4 View

My WRF simulation aborts after few minutes, why??

I am completely new to WRF, I am trying to ingest sub tiling option (mosaic approach) in WRF and the simulation aborts after few minutes. What should I do? I am attaching my namelist.input...

16 January 2019 6,111 3 View

How to induce mosaic approach (subtiling) instead of dominant approach in WRF modelling?

Please help me with this topic, I made few changes in namelist.input, the model runs for few minutes and aborts. I am completely new to this topic. Please help me out. Many Thanks. Govind Sourabh

09 January 2019 3,144 3 View

How to avoid Gaussian 09 .rwf file?

I am trying to perform some optimization calculation with G09 with PM6 semi empirical method. However, the *.rwf file is taking huge amount hard disk memory. So, it is possible to minimize/avoid...

03 January 2019 4,098 8 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View