Abdou Nagy

3 Questions 26 Answers 0 Followers

Questions related from Abdou Nagy

Is there any commercial transfection reagents suitable with large-sized plasmids?

I have succeeded recently to clone necessary elements for rAAV production into ONE universal plasmid. However, the size of this universal plasmid is 22 Kb. I am wondering if there is any...

07 March 2023 9,312 4 View

Can I store my cells for few days at 4 C before FACs analysis?

I want to ask if it is possible to store my cells 24 hours post transfection at 4 C for 4-5 days (Non-fixed) just in normal media as one of my conjugated antibody shipment is delayed and will be...

11 July 2019 5,182 4 View

What is the best purification method for removal of egg proteins and concentrating NDV virus?

I am preparing a recombinant Newcastle disease virus vaccine in fertile SPF eggs. I am going to test my vaccine candidate in guinea pigs. I want to remove egg proteins and concentrate the...

18 April 2017 2,899 2 View