Abdou Nagy

4 Questions 30 Answers 0 Followers

Questions related from Abdou Nagy

Is there any commercial transfection reagents suitable with large-sized plasmids?

I have succeeded recently to clone necessary elements for rAAV production into ONE universal plasmid. However, the size of this universal plasmid is 22 Kb. I am wondering if there is any...

07 March 2023 9,380 4 View

Can I store my cells for few days at 4 C before FACs analysis?

I want to ask if it is possible to store my cells 24 hours post transfection at 4 C for 4-5 days (Non-fixed) just in normal media as one of my conjugated antibody shipment is delayed and will be...

11 July 2019 5,274 4 View

What is the best purification method for removal of egg proteins and concentrating NDV virus?

I am preparing a recombinant Newcastle disease virus vaccine in fertile SPF eggs. I am going to test my vaccine candidate in guinea pigs. I want to remove egg proteins and concentrate the...

18 April 2017 2,957 2 View

Will I face any problem in digestion If I did not add extra bases upstream restriction enzymes of my synthesized genes?

Adding extra bases varies according to the kind of restriction enzymes you use. Many scientists advise to add 3-6 nucleotide upstream enzyme recognition sequence. If I did not add extra bases,...

20 December 2016 4,901 4 View