How can I convert microscopic image length in pixel to um for an image taken from Leica DM2500?

I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.

30 September 2020 7,979 4 View

What are the good and bad things of consuming mass gainer by a male after working out in a Gym?

Some people consumes mass gainer after working out in Gym. What are the good and bad effects of mass gainer?

13 March 2019 5,274 1 View

Which is the white cover of Artocarpus seeds?

In Artocarpus heterophyllus seeds a white coat is covering it . Can you say the actual name of the cover ? Is it endocarp or any other part ?

13 May 2018 3,730 5 View

Can I run a native gel from frozen protein lysates?

29 January 2017 4,802 4 View

How can we standardize the concentration of DNA for qPCR?

Srinath Mote

03 January 2017 8,118 3 View

What causes a stinking smell in CO2 incubator ?

24 July 2016 7,438 3 View

For how long time plant extracts can be stored?

I have prepared some methanol extracts of Rauvolfia root for alkaloid estimation. It is stored in refrigerator and now I want to know the storage life of that extract.

10 March 2016 4,700 10 View

Can anybody suggest a software to locate a set of selected SSR markers in the genome of rice and check whether these are located in genic region?

I have a set of RM series SSR markers of rice (nearly 100 in number) and I wish to check: 1. Their position in the genome at a single go (preferably with graphical output) 2. If any of these...

16 March 2015 5,831 7 View

What are the major differences between MDA-MB-231 and MCF-7 cell lines?

Please let me know why MDA-MB-231 cells are more sensitive to any drug treatment when compared to MCF-7 cells? Both of them are human breast cancer cell lines.

08 March 2015 7,189 28 View

Are there any specific primers for the molecular variability study of Rhizoctonia solani 1-1 AG?

19 December 2014 1,064 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View