How can I convert microscopic image length in pixel to um for an image taken from Leica DM2500?

I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.

30 September 2020 7,979 4 View

What are the good and bad things of consuming mass gainer by a male after working out in a Gym?

Some people consumes mass gainer after working out in Gym. What are the good and bad effects of mass gainer?

13 March 2019 5,274 1 View

Which is the white cover of Artocarpus seeds?

In Artocarpus heterophyllus seeds a white coat is covering it . Can you say the actual name of the cover ? Is it endocarp or any other part ?

13 May 2018 3,730 5 View

Can I run a native gel from frozen protein lysates?

29 January 2017 4,802 4 View

How can we standardize the concentration of DNA for qPCR?

Srinath Mote

03 January 2017 8,118 3 View

What causes a stinking smell in CO2 incubator ?

24 July 2016 7,438 3 View

For how long time plant extracts can be stored?

I have prepared some methanol extracts of Rauvolfia root for alkaloid estimation. It is stored in refrigerator and now I want to know the storage life of that extract.

10 March 2016 4,700 10 View

Can anybody suggest a software to locate a set of selected SSR markers in the genome of rice and check whether these are located in genic region?

I have a set of RM series SSR markers of rice (nearly 100 in number) and I wish to check: 1. Their position in the genome at a single go (preferably with graphical output) 2. If any of these...

16 March 2015 5,831 7 View

What are the major differences between MDA-MB-231 and MCF-7 cell lines?

Please let me know why MDA-MB-231 cells are more sensitive to any drug treatment when compared to MCF-7 cells? Both of them are human breast cancer cell lines.

08 March 2015 7,189 28 View

Are there any specific primers for the molecular variability study of Rhizoctonia solani 1-1 AG?

19 December 2014 1,064 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View