How can I convert microscopic image length in pixel to um for an image taken from Leica DM2500?

I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.

30 September 2020 7,979 4 View

What are the good and bad things of consuming mass gainer by a male after working out in a Gym?

Some people consumes mass gainer after working out in Gym. What are the good and bad effects of mass gainer?

13 March 2019 5,274 1 View

Which is the white cover of Artocarpus seeds?

In Artocarpus heterophyllus seeds a white coat is covering it . Can you say the actual name of the cover ? Is it endocarp or any other part ?

13 May 2018 3,730 5 View

How can we standardize the concentration of DNA for qPCR?

Srinath Mote

03 January 2017 8,118 3 View

For how long time plant extracts can be stored?

I have prepared some methanol extracts of Rauvolfia root for alkaloid estimation. It is stored in refrigerator and now I want to know the storage life of that extract.

10 March 2016 4,700 10 View

Is there any technique which give instant result about the plasmid transformation in host system?

As we conclude the result of transformation after overnight growth, how could we come over with Instant screening technique.

02 September 2015 2,250 3 View

Do you know a valid questionnaire for socio cultural factors influencing entrepreneurship?

18 August 2015 2,403 10 View

What are the major differences between MDA-MB-231 and MCF-7 cell lines?

Please let me know why MDA-MB-231 cells are more sensitive to any drug treatment when compared to MCF-7 cells? Both of them are human breast cancer cell lines.

08 March 2015 7,189 28 View

Are there any specific primers for the molecular variability study of Rhizoctonia solani 1-1 AG?

19 December 2014 1,064 2 View

How can I get the crystallographic card of Cloisite 93 A ( a modified Montmorillonite nanoclay) & epoxy/cloisite nanocomposites?

Crystallographic card (PDF) JCPDS indexes

14 September 2014 2,036 5 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View