I there any data on the monthly rainfall in the Southern Western Ghats during 2011 and 2012?
I am working on a grignard reagent(PhMgBr) addition reaction(with alpha,beta unsaturated ketone) and my product is a tertiary alcohol. I have found through Tlc, I had got a mixture.How to purify...
21 April 2024 8,582 0 View
can any one explain me the procedure for the preparation of tertiary alcohols by the addition of ketone to the Grignard reagent, so that i can get the pure product. Also please suggest me the best...
21 April 2024 5,207 0 View
What is the maximum yield of colanic acid has been produced? and What is the best method of producing Colanic acid? Tell me the process, production, purification, characterization, and removal...
23 March 2024 9,032 0 View
Like in Lung cancer how b cells are getting affecting, so just want to how and what are the complications B cells are facing regarding different type of cancerous cells
06 December 2023 6,132 1 View
I used fullprof software fo the refinement of xrd data. Is there any way to know the density from the software.
16 January 2023 2,965 1 View
I read one or two articles about systematic reviews conducted in descriptive/ epidemiological studies, but those articles were published in 2000 and 2010 respectively. So I want to be sure about...
11 September 2022 4,290 2 View
In xrd I got crystalline peak at 28.65. Whether this peak corresponds to graphite?
11 March 2022 8,633 4 View
I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.
30 September 2020 8,201 4 View
Mycorrhiza is a obligate symbionts so it can not grow without root. I found it in many research article.
04 September 2020 5,242 5 View
here I wanted to study each dimension of marketing efforts that online retailers use to reduce customers perceived risk categories as follows; Financial marketing efforts like COD, payment...
25 July 2020 8,697 5 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
02 July 2024 8,715 2 View
I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...
01 July 2024 7,104 1 View
I am working on one protein which gets aggregated (similar to alpha-synuclein and tau) in certain neurodegenerative diseases. I am trying to confirm the aggregation using Western blotting....
30 June 2024 5,060 3 View