How can I convert microscopic image length in pixel to um for an image taken from Leica DM2500?

I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.

30 September 2020 7,979 4 View

What are the good and bad things of consuming mass gainer by a male after working out in a Gym?

Some people consumes mass gainer after working out in Gym. What are the good and bad effects of mass gainer?

13 March 2019 5,274 1 View

Which is the white cover of Artocarpus seeds?

In Artocarpus heterophyllus seeds a white coat is covering it . Can you say the actual name of the cover ? Is it endocarp or any other part ?

13 May 2018 3,730 5 View

How can we standardize the concentration of DNA for qPCR?

Srinath Mote

03 January 2017 8,118 3 View

For how long time plant extracts can be stored?

I have prepared some methanol extracts of Rauvolfia root for alkaloid estimation. It is stored in refrigerator and now I want to know the storage life of that extract.

10 March 2016 4,700 10 View

What are the major differences between MDA-MB-231 and MCF-7 cell lines?

Please let me know why MDA-MB-231 cells are more sensitive to any drug treatment when compared to MCF-7 cells? Both of them are human breast cancer cell lines.

08 March 2015 7,189 28 View

Are there any specific primers for the molecular variability study of Rhizoctonia solani 1-1 AG?

19 December 2014 1,064 2 View

How can I get the crystallographic card of Cloisite 93 A ( a modified Montmorillonite nanoclay) & epoxy/cloisite nanocomposites?

Crystallographic card (PDF) JCPDS indexes

14 September 2014 2,036 5 View

mean fluorescence intensity

what is mean fluorescence intensity? what is its use and how it is measured?

08 August 2011 4,102 18 View

What is the difference between predictive & model predictive control?

Dear Sir, I have read few articles about predictive torque control of an Induction machine. Is there any specified difference in designing or control aspects between predictive & model...

01 January 1970 9,279 7 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

I want to remove TiO2 and SiO2 coating from surface. any one can help me?

I want to remove TiO2 and SiO2 coating from surface. I think that i should use HCl and MgCl2 and an oxidation agent such as potassium nitrate, but i dont know in which propertion should these...

14 February 2021 300 3 View

What is the maximum wavelength and absorbance for dodecyl trimethylammonium bromide?

I am measuring the absorption of dodecyl trimethylammonium bromide surfactant using UV spectrometer. I am trying to set the wave length range for the experiment. Please does anyone have a idea of...

07 February 2021 7,720 1 View

References for relative humidity created by salt solutions at different temperatures?

I understand that the ability of standard salt solutions commonly used to produce a certain relative humidity (RH) in a closed space changes somewhat with the temperature. Where do I find data...

04 February 2021 7,370 4 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View

How to recrystallization amine compound and it is not soluble in common organic solvents ?

How to recrystallization amine compound and it is not soluble in common organic solvents ?

19 January 2021 671 3 View

For a PCR the volume of each component is standard ?

for example the total volume is 50microliters how can we know that what will be the amount of template , buffer, enzyme, primers (forward and reverse), dNTPs, MgCl2 and water?

02 January 2021 3,352 6 View