What are the problems for gentamicin derivatization?

18 November 2015 1 982 Report

I'm working on controlled release of gentamicin from microspheres. To measure the drug amount I use derivatization method which includes ortho-phthalaldehyde, beta-mercaptoethanol, methanol in 0.04M sodium tetraborate. But after derivatization, I couldn't obtain any measurement at 333 nm neither peak. What is the problem that I did wrong?

Bikash Debnath

Gentamicin is the cheapest and the first line aminoglycoside antibiotic. Intake the Gentamicin for serious gram negative bacillary infection it has gives relatively more nephrotoxic effect in human.

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