What is the best media/solution for preservation or transfer of samples from the gingiva for bacterial identification and PCR? is TE buffer enough?

More Inas Alhudiri's questions See All

In bibliometric analysis, which methodology is more suitable: framework research or conceptual research?

To conduct a bibliometric analysis for a review paper in economic or social science, it is crucial to select the appropriate structure to address the research question. Among the available...

27 April 2024 497 9 View

How many pre test samples are needed?

HI Wanted to know what is the best sample size for a non probability pre test Questionaire. what is recommended for a minimum sample size of 500?

27 February 2022 1,793 5 View

What the Scientific reason that cause fast spreading of corona virus through short time?

Despite there are many viruses, bacteria, germs, nanomaterial in atmosphere, but corona virus can be spreading fastly

29 December 2020 4,042 9 View

Carboxylic acids..chemists supposed to be here?

I have to find carboxyl acids quantitatively via basic calculation except any experimental study such as titration etc. How can I calculate the amount of carboxylic acid groups in polymer chains?

19 March 2016 8,249 8 View

What are the problems for gentamicin derivatization?

I'm working on controlled release of gentamicin from microspheres. To measure the drug amount I use derivatization method which includes ortho-phthalaldehyde, beta-mercaptoethanol, methanol in...

17 November 2015 1,009 1 View

I am getting extremely low counts of Droplets with Digital PCR (Biorad), Any clue why? Any tricks?

My Droplets Count range is between 1000-6000

23 July 2015 3,301 7 View

Can anyone help with transfecting immortalized cornea epithelial cells with miRNA mimic using lipfectamine?

Hi All, Do you add the lipofectamine miRNA mix to the media which the cells are already in or remove the media and just add the lipfectamine-mimic mix in OPti-MEM media. And I also need to know...

23 June 2015 1,299 2 View

Hi All, What would I use to dilute trypsin from 0.25% to 0.05% for splitting? Should I use sterile PBS or serum free media?

I am using Cornea epithelial cells from ATCC

07 June 2015 8,410 9 View

What method is most suitable for water annealing for film preparation using a desiccator?

What is the main idea of water annealing process on film formation by using desiccator? In your opinion is water or methanol the best one for increasing insolubility?

03 March 2015 8,106 3 View

Why is there is no peak when dissolving hederagenin in methanol by HPLC?

Best method for hederagenin saponin analysis on HPLC/UV.

09 January 2014 1,073 4 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View