Who experience on SDS-PAGE with a small peptide?

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What procedure should I follow for current controlled PPy film electrochemical deposition on ITO?

For example, I perform passivation process before electropolymerization of Py for adhesion to titanium surface. Is a similar or different procedure required for ITO? If necessary, I would be glad...

06 August 2024 3,176 0 View

Does the specific capacitance value decrease or increase over time?

Will these measurement results change, for example, 1 month after the first measurement (e.g. CV, EIS) of the electrode or supercapacitor device? If it changes, how will it change? Is there an...

03 July 2024 3,644 2 View

Why are acids such as sulfuric and phosphoric used in gel electrolytes used in supercapacitors?

While the purpose of adding acid types into the PVA plasticizer when making gel electrolytes in supercapacitors is for conductivity, what are the other purposes? So why do we add acid? Why do we...

07 May 2024 6,320 2 View

What is the way to adjust GCD potential?

I could not manage to fall into negative potential while taking GCD on Versastad 4 potentiostad. So if the CV is between -0.5 AND 1.0 V, I want to use the same potential range in the GCD. I enter...

24 April 2024 4,163 0 View

Is it possible to write a PDB file inside an NAMD configuration file?

I'm doing several steps, such as minimization and equilibration, to start running MD simulations and I'm trying to automate this process by running one step after finishing the other. When I go...

10 February 2024 7,714 1 View

For supercapacitor electrodes obtained from graphite powder, what kind of material is used to bond / combine the powders?

For supercapacitor electrodes obtained from for example graphite powder, what kind of material is used to bond / combine the powders?

05 February 2024 7,809 2 View

How to chemical deposition of pyrrole dope with sulphuric acid on graphite powder?

Which steps, materials, temperature or stirring contain this process? Is there any publish or book? I want to materials make up only sulphuric acid and pyrrole monomer. Because my materials are...

01 February 2024 1,386 2 View

Can you recommend a publication or a book that does EIS and Mott Schottky analysis in the combined use of polymers and metal oxides?

Hello everyone, Can you recommend a publication or a book that does EIS AND Mott Schottky analysis in the combined use of polymers and metal oxides? I need books, publications, or any other...

17 December 2023 900 2 View

Resist developer etching time for ALD films?

Hi, Does anyone has experience with resist developer etching time for ALD films? My...

11 November 2023 6,646 0 View

How is pyrrole doped with sulfuric acid on Al substrate or Cu band produced by electrodeposition?

Hello, I made passivation on the Cu tape by just waiting in NaOH, it was necessary to coat it with a film of sulfuric acid and pyrrole monomer. But when an acidic polymer entered the solution, the...

24 August 2023 3,277 1 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View