Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

Does anyone know why hela cells change shape and look like more fibroblast?

Hello everyone. In my experiment in 24wp some of the wells look like more fibroblast than hela. I used RPMI with 10% FBS and no antibiotics. Do I have a contamination? or are my cells dead? The...

27 March 2018 6,821 3 View

Does any one know Chemical Analysis for Gradient Grade HPLC solvents ?

12 November 2016 7,298 4 View

How long oligopeptites can be precipitation by TCA ?

Proteins can be precipitated by TCA, can tripeptides, pentapeptides, hekzapeptides and other oligopeptites can be precipitated by TCA ? What is the amino acid limit ?

23 April 2016 8,114 3 View

Carboxylic acids..chemists supposed to be here?

19 March 2016 8,123 8 View

What method is most suitable for water annealing for film preparation using a desiccator?

What is the main idea of water annealing process on film formation by using desiccator? In your opinion is water or methanol the best one for increasing insolubility?

03 March 2015 7,979 3 View

How can we distinguish transconjugant cells from recipient cells in minimal agar medium?

03 December 2014 7,892 4 View

What are the factors affecting the adhesion strength between metal/oxide and metal/nitride layers?

Microstructure, roughness, the enthalpy of formation of an oxide compared to the adjacent layer are some of the factors affecting the adhesion strength. Is there a specific variable for...

25 September 2014 549 7 View

What is the weight of a vertically aligned carbon nanotube?

How can I weigh the vertically aligned CNT? I want to prepare a supercapacitor cell to measure capacitance, but I could not find any easy way to measure it.

21 May 2014 2,667 1 View

Does anyone know an HPLC method for determination of AESCIN? I want to analyzie assay of aescin in a cream sample on HPLC..

Aescin HPLC

27 February 2014 1,397 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View