What are the possible genotyping methods for Acinetobacter spp.? In other words, what are the possible interspecies genotyping methods for different Acinetobacter spp.?
I'm not aiming for identification at this point but only for typing isolates into species-specific groups.
One that I know is the amplification of the 16S rRNA genes by PCR followed by RFLP.
Are there other methods with better discriminatory Power?
In our lab, we use rep-PCR fingerprinting as a simple, rapid, and inexpensive method for typing a wide range of bacteria from various sources. I don't have experience with Acinetobacter spp. in particular, and I'm not sure how specific the method would be in this case. With some bacteria, the method can be used for rough identification if you include reference strains in your fingerprint database.
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