Mahmoud Gharieb

14 Questions 5 Answers 0 Followers

Questions related from Mahmoud Gharieb

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

06 August 2024 6,651 4 View

Can heterogeneous resistance affect the zone diameter in disk diffusion susceptibility testing?

Can heterogeneous resistance, indicated by the presence of small discrete colonies inside the zone of inhibition, affect the zone diameter in disk diffusion susceptibility testing?

30 March 2024 2,712 3 View

Do mutations caused by serial passage of bacterial strains in culture media depend on the type of the media?

"Serial passage of bacteria can result in increased genomic diversity of derivative strains relative to the parental strain" In Vitro Serial Passage of Staphylococcus aureus: Changes in... Is...

23 January 2024 8,572 12 View

Can wrinkled colony morphology variants of gram negative bacteria have false negative KOH string test?

From the practical perspective, anybody encountered this before? From the theoretical perspective, is this there any correlation possible?

03 January 2024 4,788 0 View

Can Gravity convection incubators negatively affect the growth of strict aerobic bacteria?

I am currently studying strict aerobic bacteria and encountering challenges with the growth rate in broth cultures. Despite using an inoculum size of 4-5 colonies, a low broth volume (4-5 ml), and...

08 December 2023 6,849 7 View

What are the colony morphology characteristics influenced by non genetic factors for monogenetic bacterial colonies on the same plate?

I'm trying to figure out the colony morphology characteristics that one should be skeptical of when deciding whether two colony types on the same plate represent morphologic variations of a single...

01 October 2023 4,577 4 View

How do i figure out whether a recognition site is conserved or not, in an automated way?

"Restrict from EMBOSS Suit version 6.3.1 with the following parameters: snucleotide1, sitelen = 4, rformat = table, enzymes = enzymes.txt. From the 4379 enzymes present in REBASE, we selected the...

24 April 2023 6,787 4 View

What is the potential for future variability in conserved restriction sites due to being within variable regions?

"The result shows absence of intragenomic variation among 16S rDNA gene and presence of variable regions among the 16S rDNA sequences (intergenomic variation), noticing for example high...

15 April 2023 6,288 3 View

How can you perform D-glucose assimilation test for a bacterial strain without API kit?

Facts: Glucose assimilation test is based on the ability of a bacterial species to utilize glucose as a sole carbon source. The test is designed to determine whether the organism can use glucose...

25 March 2023 7,570 0 View

Why is a "final extension" step needed in PCR for ARDRA?

Facts: Amplified ribosomal DNA restriction analysis (ARDRA) involves the amplification of the 16S rRNA gene (1500 bp) followed by enzymatic restriction and gel electrophoresis. The terminal...

03 March 2023 2,931 1 View

What are the possible non-sequencing based genotyping methods for Acinetobacter spp.?

What are the possible genotyping methods for Acinetobacter spp.? In other words, what are the possible interspecies genotyping methods for different Acinetobacter spp.? I'm not aiming for...

31 December 2022 5,104 3 View

Is there any mention whether the path to obesity or obesity itself, what leads to obesity related chronic diseases?

What i mean by the path to obesity, is the type of excess calories that led to this obese state: someone can get obese on a ketogenic diet (very low carbohydrate) Vs. someone on a low fat high...

04 November 2022 4,395 2 View

Why are beta-lactam antibiotics the most common drug allergens?

β-Lactam antibiotics: the most common allergens among drugs. doi: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692812/ Why are beta-lactam antibiotics the most common drug allergens? I was...

20 October 2022 8,277 3 View

What is the meaning of typability in bacterial strain typing?

"Typability: the ability of the method to produce an unambiguous result for all strains of a particular species" [Detecting Pathogens in Food, T A McMeekin, Elsevier, Jun 23, 2003] What is an...

15 October 2022 4,091 4 View