Mahmoud Gharieb

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Questions related from Mahmoud Gharieb

How do i figure out whether a recognition site is conserved or not, in an automated way?

"Restrict from EMBOSS Suit version 6.3.1 with the following parameters: snucleotide1, sitelen = 4, rformat = table, enzymes = enzymes.txt. From the 4379 enzymes present in REBASE, we selected the...

24 April 2023 6,773 4 View

How can you perform D-glucose assimilation test for a bacterial strain without API kit?

Facts: Glucose assimilation test is based on the ability of a bacterial species to utilize glucose as a sole carbon source. The test is designed to determine whether the organism can use glucose...

25 March 2023 7,559 0 View

Why is a "final extension" step needed in PCR for ARDRA?

Facts: Amplified ribosomal DNA restriction analysis (ARDRA) involves the amplification of the 16S rRNA gene (1500 bp) followed by enzymatic restriction and gel electrophoresis. The terminal...

03 March 2023 2,918 1 View

Why are beta-lactam antibiotics the most common drug allergens?

β-Lactam antibiotics: the most common allergens among drugs. doi: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692812/ Why are beta-lactam antibiotics the most common drug allergens? I was...

20 October 2022 8,266 3 View