We use RPMI 1640 medium supplemented with 10% fetal bovine serum (Invitrogen, Paisley, UK), 2mM L-Glutamine (Invitrogen); 100U/ml penicillin and 100µg/ml streptomycin (Sigma-Aldrich).Culture the cells at 1x10^6 per ml of media. We dont use HEPES or sodium bicarbonte
We use RPMI 1640 medium supplemented with 10% fetal bovine serum (Invitrogen, Paisley, UK), 2mM L-Glutamine (Invitrogen); 100U/ml penicillin and 100µg/ml streptomycin (Sigma-Aldrich).Culture the cells at 1x10^6 per ml of media. We dont use HEPES or sodium bicarbonte