I want to prepare perfusion buffer consisting of:
collagenase B (2.5 mg/ml),
Dispase II (1.2 U/ml; Roche),
Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma),
DNase I (LS002058, 125 Kunitz/ml; Worthington)
I have ordered all the enzymes needed, but I am struggling to prepare the proper concentrations of each.
and do I need to aliqout?
Can you please help me?