I want to prepare perfusion buffer consisting of:

collagenase B (2.5 mg/ml),

Dispase II (1.2 U/ml; Roche),

Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma),

DNase I (LS002058, 125 Kunitz/ml; Worthington)

I have ordered all the enzymes needed, but I am struggling to prepare the proper concentrations of each.

and do I need to aliqout?

Can you please help me?

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