Ioanna Eleftheriadou

7 Questions 20 Answers 0 Followers

Questions related from Ioanna Eleftheriadou

How can i check if there is active efflux system in clinical isolates?

I want to test some natural products as efflux pump inhibitors in MDR bacteria. I have already done the ethidium bromide-agar cartwheel method (with some modifications) on clinical samples of...

30 March 2018 7,333 3 View

How can i dilute carvacrol (oil) ?

I have read in some papers that carvacrol can be diluted in Tween 80 (1%) and distilled water. I have tried this but carvacrol wasnt fully dissolved. After that i tried to dissolve carvacrol in...

10 August 2016 6,183 7 View

Can anybody help with MTT assay in PBMC ?

I would like to evaluate the cytotoxicity of a compound in PBMC with a MTT assay. I add 100μl of cell suspension(seed 100.000 cells/well) and 100μl extract and after time of incubation i add 20μl...

21 April 2016 5,263 25 View

Is a standard curve required for each assay when we use an elisa kit?

Does anybody know if i can use some wells ( about 10 wells)of the TNF-a elisa kit in order to try my samples? I have not used a kit before and i wonder whether i can use some wells without running...

26 March 2016 5,439 17 View

Can I use blood that has been diluted in RPMI (supplemented with 10%FBS and 5ml penicillin/streptomycin) for isolation of PBMC using biocoll-1.077 ?

I collected the blood sample(20ml) and i used 10 ml of blood diluted in 10 ml pf PBS.I diluted the rest of the blood with RPMI (supplemented with 10%FBS and 5ml penicillin/streptomycin) and i...

16 February 2016 768 5 View

Can someone help me find out the recommended carvacrol and thymol concentrations in vitro?

We are testing the anti-inflammatory activity of these compounds on human PBMCS in vitro. Is there any recommended concentrations that are being used? I would be grateful for some references in...

20 January 2016 180 3 View

What kind of cells should I to use in order to study the process of inflammation ?

I'm going to study the anti-inflammatory effects of carvacrol and thymol in vitro using ELISA kits for TNF-a and IL-6. I have to use only monocytes? I'm going to use cells from patients with...

01 December 2015 7,597 12 View