I am using 50-100 ng/ml PMA but my cells won't phagocyte Candida albicans and they aren't adherent to the plastic dish (almost 60% are a bit adherent).
I am seaching for a software that would enable me to create a database constitued of PDF files of older ethnographies of north american indigenous peoples. Here I'm thinking of ethnographies like...
19 January 2021 8,714 5 View
Our lab recently inherited an Aquamax DW4 plate washer. This device is no longer supported/serviced by Molecular Devices (manufacturer). It did not come with the CD-ROM and we are wondering if...
10 June 2020 8,650 0 View
This post is going to be a little long, so I apologize in advance for bothering you. I learned that quality control is super important so I want to make sure that I do this right. I have several...
18 May 2020 6,670 1 View
I have a set of buffers (Tris, imidazole, phosphate) for NI-NTA protein purification. I prepared them about three weeks ago and they contain DTT and PMSF. Can I add fresh DTT and PMSF to the...
03 July 2019 2,670 3 View
Imagine we had two subject groups: controls and patients with a certain condition, altering lipidomes of their blood. Then we obtained abundances of each molecular species for both groups and...
05 February 2018 8,386 1 View
Dear all, I deposited CrN film using different conditions. I observed a strong texturation along (111) direction (already reported in numerous publication), but a large variation of the films...
03 January 2018 5,236 3 View
Hello everyone, I want to know how can i be updated on the hot papers/articles in soil science, soil pollution,... any help i will appreciate Thanks a lot for your replies.
24 August 2017 5,601 10 View
In Madagascar island, 6 unknown eaten pleurotoid occur in native humide forests or plants material wastes.
16 May 2017 1,240 2 View
I am extracting RNA from FFPE tissue (Phenol Chloroform method). I am not being able to achieve complete homogenization with the tissue homogenizer. I was thinking about using sonication. Do you...
17 December 2016 254 1 View
Can We Detect Acute Ischemic Stroke (AIS) known as Brain Stroke Episode based on ECG-Heart Signal at an Earlier Stage of Time? So far we can Detect ST Bio-Markers and the Behavior of the T-wave of...
15 November 2016 7,378 5 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View