Are you or other searchers interested to extend the same study on Pleurotus spp of Madagscar ?

More Emile Randrianjohany's questions See All

What would be the best way to create an ethnographic database in order to facilitate comparative work ?

I am seaching for a software that would enable me to create a database constitued of PDF files of older ethnographies of north american indigenous peoples. Here I'm thinking of ethnographies like...

19 January 2021 8,714 5 View

Aquamax DW4 Plate Washer Software?

Our lab recently inherited an Aquamax DW4 plate washer. This device is no longer supported/serviced by Molecular Devices (manufacturer). It did not come with the CD-ROM and we are wondering if...

10 June 2020 8,650 0 View

What is the best way to do QC with plink ?

This post is going to be a little long, so I apologize in advance for bothering you. I learned that quality control is super important so I want to make sure that I do this right. I have several...

18 May 2020 6,670 1 View

Can I add fresh DTT and PMSF to previous buffers?

I have a set of buffers (Tris, imidazole, phosphate) for NI-NTA protein purification. I prepared them about three weeks ago and they contain DTT and PMSF. Can I add fresh DTT and PMSF to the...

03 July 2019 2,670 3 View

Lipid abundances to enzyme pathways?

Imagine we had two subject groups: controls and patients with a certain condition, altering lipidomes of their blood. Then we obtained abundances of each molecular species for both groups and...

05 February 2018 8,386 1 View

Relation between film density / texturation / bandgap ?

Dear all, I deposited CrN film using different conditions. I observed a strong texturation along (111) direction (already reported in numerous publication), but a large variation of the films...

03 January 2018 5,236 3 View

Hot papers in soil science or soil polution ?

Hello everyone, I want to know how can i be updated on the hot papers/articles in soil science, soil pollution,... any help i will appreciate Thanks a lot for your replies.

24 August 2017 5,601 10 View

Does tissue lysis by sonication affect the RNA integrity?

I am extracting RNA from FFPE tissue (Phenol Chloroform method). I am not being able to achieve complete homogenization with the tissue homogenizer. I was thinking about using sonication. Do you...

17 December 2016 254 1 View

Does Qiazol enables the extraction of proteins and DNA from the same sample along with RNA extraction?

I want to extract proteins and RNA from tissue samples. TRIzol reagent is not available in our labs. We have Qiazol instead? Does it do the same job? Are these products comparable in efficiency?...

13 October 2016 778 3 View

Can we stain fission yeast using Calcein-AM?

Can we "live stain" S. Pombe using Calcein-AM/Hoeschst (plasma membrane and cell wall as well as the septum and nucleus)?

06 July 2016 1,974 0 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Can anyone share a protocol for ROS assay using DCFDA dye for insect tissues?

I am trying to estimate ROS in insect tissue. Is it possible to do it using DCFDA

18 June 2024 6,954 0 View

Which primer should be selected for DNA barcoding of jellyfish for the species identification?

For the identification of jellyfish, mCOI, ITS1-2, 16S, 18S, and 28S primers have been used in different published research papers. Among them, I am considering selecting the primers mCOI and...

11 June 2024 4,001 5 View

ROS-Glo kit (Promega) measures high blank, negative and solvent control in spheroids, any advice?

We are trying to measure intracellular H2O2 in spheroids post exposure to nanozymes, that should generate ROS, using the ROS-Glo kit from Promega. However, we are getting strange results. The...

09 June 2024 2,754 0 View

What is the mechanism of reactive oxygen species (ROS) generation in bacteria? Is it similar with plants ROS generation machinery?

For example, this article https://www.mdpi.com/2079-7737/11/2/155

09 June 2024 897 0 View

How to detect mitochondrial ROS production in BMDM using mitoSOX red?

Hi everybody! I got some strange results when I tested mitoSOX RED on Bone marrow-derived Macrophages. According to the publications, bone marrow-derived macrophages(BMDMs) generally show...

03 June 2024 9,853 1 View

Why is it that I keep on obtaining negative and positive %radical scavenging activity (RSA) in DPPH?

As a context, we have been optimizing the DPPH protocol for our seaweed ethanolic extract. We also use varying concentration points from the various methods in published journal articles, yet none...

28 May 2024 3,097 2 View

Could anyone help me identify the species?

Could anyone help me in identification of these species growing in China's coastal wetlands? I attach some photos. Thank you.

27 May 2024 9,656 7 View