Why Seminomas spread mainly via lymphatic route and NSGCT with Haematogenous route?

What is the reason of such specific orientated route of transmission?

01 January 1970 7,637 1 View

What is the reason of 'alcohal'fastness of Tuberculosis?

Mycolic acid is responsible for acid fastness. Mycolic acid by nature is a lipid rich material which must be washed when bacteria is treated with alcohal. Then what makes it alcohal fast?

01 January 1970 7,141 1 View

Why do Ricketssial rashes spare face,palm,sole? Mechanism behind it?

Ricketssia affect endothelium and due to its damage it causes maculopapular rashes but why they doesn't happen on face,sole,palms?

01 January 1970 9,203 4 View

Why primary viral pneumonia has a predilection for individuals with cardiac diseases, particularly mitral stenosis?

What is the reason of this predilection?

01 January 1970 9,739 2 View

In G6PD deficiency why we talk only about damage to rbc? Other cells should have deficiency of the same enzyme increading oxidative stress to them?

Do G6PD deficiency harmfull for all cells? Why we only talk about rbc when it comes to G6PD deficiency?

01 January 1970 7,837 6 View

What are the reasons of prolapse of Facial nerve over stapes?

Facial nerve prolapse reasons?

01 January 1970 1,378 3 View

Where i can find the etymology of drugs in pharmacology? Any site regarding that?

How are names given to drugs? Is there any online site where i can get to know the origin of name of the drugs?

01 January 1970 6,320 5 View

When we say inhibition of enzyme cytochrome p450 what do we really mean?

How do a drug inhibit a metabolising enzyme? Is it due to decreased expression of gene producing enzyme?

01 January 1970 9,693 4 View

No title

Why fungus change its morphology with temprature and how is this really happening?

01 January 1970 2,382 3 View

Why it is said that glomeruli are relatively resistant to infections?

Is it due to high pressure blood that do not allow microbial growth there?

01 January 1970 3,606 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View