Which organic solvent should be used for liquid-liquid extraction of water soluble azo dyes for GC-MS and FTIR analysis?

I have used ethyl acetate (the most reported solvent for extracting dyes and their degraded products) but the problem is i am unable extract the original dye solution, can any body suggest me some...

01 February 2016 3,288 1 View

What is the preferable method for sample preparation of degraded dye (mixture) samples/metabolites for performing the GC-MS analysis?

What is the preferable method for sample preparation of degraded dye (mixture) samples/metabolites for performing the GC-MS analysis? Most of the available literature is on individual dyes. Could...

08 September 2015 422 4 View

What is the preferable method for total DNA extraction from enriched broth culture samples?

I am looking for a protocol for total DNA extraction from enriched broth samples (enriched from soil and sludge samples). Could the methods available for DNA extraction from soil samples be used...

07 August 2015 9,473 3 View

Can anyone tell me whether Placket-Burman or Central composite design is most suitable for carrying out optimization experiments?

i am working on textile dyes' degradation for which i have to optimize different process parameters (atleast 8 factors). I have reviewed the literature and found both of these designs in different...

05 June 2015 2,611 5 View

Which is best the best procedure for obtaining pure cultures of protozoans e.g. amoeba, paramecium?

11 December 2014 6,418 6 View

What is the best procedure for sample preparation for FTIR analysis of degraded textile dye samples i.e. in simulated and real effluent?

I have used liquid samples earlier but the results were not good, due to the major peak of OH group, all other peaks become inconspicuous and thus difficult to interpret. The other alternative...

10 November 2014 9,265 6 View

Which type (material) of filter/syringe filter is suitable for filtration of azo dyes?

See above

09 October 2014 4,635 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View