I have used liquid samples earlier but the results were not good, due to the major peak of OH group, all other peaks become inconspicuous and thus difficult to interpret. The other alternative which is mostly found in literature is solvent extraction using ethyl acetate or any other solvent. But again that method is not much useful as the dyes and hence their metabolite concentrations are in ppm that make the detection really hard . what should i do then?? is there any other method available or can somebody suggest me a better extraction (for sample concentration) procedure??
Extract the degraded dye with an equal volume of ethyl acetate. Dry the extract over anhydrous Na2SO4 and evaporate solvent on a rotary evaporator. The residues obtained after evaporation should be dissolved in small volume of high-performance liquid chromatography (HPLC) grade methanol and used for analytical studies. For FTIR analysis mix the samples with spectroscopically pure KBr in the ratio of 5:95 and read in mid-IR region of 400–4,000 cm-1 with 16 scan speed using Perkin Elmer 783 spectrophotometer and compared with control dye
If you have a mixture in your sample and your sample may effected during heating with rotary evaporate .I prefer to used lympholizer (freeze dryer ) and check TLC to identify your component . you can used your sample directly for FT-IR with ATR unite( no KBr disk).then you can more analysis upon your idea.
You can transfer to to organic phase (Methanol-HPLC grade) - Refer Zille et al 2005
in the extraction of dyes metabolites after bacterial degradation present in organic or aqueous layer for FTIR analysis?
the color of dyes present in aqueous extract
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