What is the preferable method for sample preparation of degraded dye (mixture) samples/metabolites for performing the GC-MS analysis?

What is the preferable method for sample preparation of degraded dye (mixture) samples/metabolites for performing the GC-MS analysis? Most of the available literature is on individual dyes. Could...

08 September 2015 422 4 View

What is the preferable method for total DNA extraction from enriched broth culture samples?

I am looking for a protocol for total DNA extraction from enriched broth samples (enriched from soil and sludge samples). Could the methods available for DNA extraction from soil samples be used...

07 August 2015 9,473 3 View

Can anyone tell me whether Placket-Burman or Central composite design is most suitable for carrying out optimization experiments?

i am working on textile dyes' degradation for which i have to optimize different process parameters (atleast 8 factors). I have reviewed the literature and found both of these designs in different...

05 June 2015 2,611 5 View

What are the different methods for differentiation between live and dead cells in protozoans?

11 December 2014 1,823 2 View

Which is best the best procedure for obtaining pure cultures of protozoans e.g. amoeba, paramecium?

11 December 2014 6,418 6 View

What is the best procedure for sample preparation for FTIR analysis of degraded textile dye samples i.e. in simulated and real effluent?

I have used liquid samples earlier but the results were not good, due to the major peak of OH group, all other peaks become inconspicuous and thus difficult to interpret. The other alternative...

10 November 2014 9,265 6 View

Which type (material) of filter/syringe filter is suitable for filtration of azo dyes?

See above

09 October 2014 4,635 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View