Which organic solvent should be used for liquid-liquid extraction of water soluble azo dyes for GC-MS and FTIR analysis?

I have used ethyl acetate (the most reported solvent for extracting dyes and their degraded products) but the problem is i am unable extract the original dye solution, can any body suggest me some...

01 February 2016 3,288 1 View

What is the preferable method for sample preparation of degraded dye (mixture) samples/metabolites for performing the GC-MS analysis?

What is the preferable method for sample preparation of degraded dye (mixture) samples/metabolites for performing the GC-MS analysis? Most of the available literature is on individual dyes. Could...

08 September 2015 422 4 View

Can anyone tell me whether Placket-Burman or Central composite design is most suitable for carrying out optimization experiments?

i am working on textile dyes' degradation for which i have to optimize different process parameters (atleast 8 factors). I have reviewed the literature and found both of these designs in different...

05 June 2015 2,611 5 View

What are the different methods for differentiation between live and dead cells in protozoans?

11 December 2014 1,823 2 View

Which is best the best procedure for obtaining pure cultures of protozoans e.g. amoeba, paramecium?

11 December 2014 6,418 6 View

What is the best procedure for sample preparation for FTIR analysis of degraded textile dye samples i.e. in simulated and real effluent?

I have used liquid samples earlier but the results were not good, due to the major peak of OH group, all other peaks become inconspicuous and thus difficult to interpret. The other alternative...

10 November 2014 9,265 6 View

Which type (material) of filter/syringe filter is suitable for filtration of azo dyes?

See above

09 October 2014 4,635 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View