Can anyone suggest a highly efficient and not toxic transfection reagent for CCF-STTG1 human astrocytoma cell line transfection?

I am using CRISPR Knockout and activation products from Santacruz biotechnology, particularly double Nickase Plasmid. I followed their protocol and tried a different ratio of DNA plasmid and...

10 November 2018 9,589 0 View

What is the most benefit: study the same branch at Master and Ph.D degrees or change them?

Today, every educational field or domain contains several branches. You first choose one branch of your field to prepare your Master and Ph.D degrees. What is preferable system for you: 1- study...

11 February 2018 8,479 30 View

What do you focus when you publish your articles: quality or quantity?

All of us have a different view of point when we prepare study on some topics. Some of us try to study only the depth results in the work, whereas other think it must to investigate all results...

18 December 2017 7,493 76 View

The good, the bad and the ugly. Any positive effect of the climate change?

Is there any positive effect of recent climate change?

31 December 2014 1,124 4 View

Is Moran's eigenvector maps (MEM) really better than principal coordinates of neighbour matrices (PCNM)?

I know that many biostatisticians prefer using MEM rather than PCNM in spatial ecology and biogeography, although the later belongs to the same MEM family. Why? I am quite familiar using PCNM but...

04 May 2013 7,684 10 View

Where should we publish or how many publications should we have? Which is better for young ecologists?

As a young ecologist, I am always looking for better knowledge to improve myself and grow up as a professional ecologist. During this period, we started applying for research funds or research...

31 December 2012 8,629 12 View

Is all journals have abbreviated names?

Some time, I could not find the abbreviated names of some journals. Is there any website contains all of them.

01 January 1970 792 12 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View