Hello,

I'm doing a retroviral transduction using the Phoenix Eco as a packaging cell line and after the transfection I collect the supernatant and then proceed to filter it as detailed in Nolan's protocol for retrovirus preparation using the Phoenix cell line ( https://web.stanford.edu/group/nolan/_OldWebsite/protocols/pro_helper_dep.html)

My question is if I can use a mixed cellulose ester membrane to filter the supernatant. (MF-Millipore Membrane, mixed cellulose esters, Hydrophilic, 0.45 µm, 293 mm, plain)

I remember reading a paper once that said that certain kinds of cellulose would retain the virus by adsorption and the viral titer would be greatly reduced, but I'm not sure if this is one of those kinds of cellulose and I can't find any specifications that say that it is not suitable for retroviral production.

Alternatively the protocol also says that spinning down and discarding the cell pellet should be enough but I've already tried this and have had a cross-contamination in the culture of target cells.

Does anyone know if the filter that I plan to use will be alright?

Thank you!