Hello,
Genotyping can be proceeded by Affymetrix...chips. Who can tell me which one is more performant? What are the differences between those chips ?
Thanks
Hello , I'm asking you why they consider that Wilcoxon test and U Mann Whitney test are the same ? Can we use this test to examine 2 numeric variables for independent groups ? Thank you
08 September 2018 8,735 3 View
Hello, I am conducting a research on the key domains of a different protein sequences. Can you give me the link of the best software that I can use to do this task ?
11 December 2017 5,865 3 View
hello, i want to know why according to the literature when they establish standard curves they use cloned bacterial DNA (for exemple for feacalibacterium strains) in a plasmid and for other...
04 May 2017 1,245 2 View
Hello, I want to sequence rRNA16S of bacteria by means of next generation sequencing technic using Illumina MiSeq technology. In articles i see that they choose hypervariable region V4 or V6...
11 December 2016 1,564 1 View
Hello, I want to compare data related to nutrition of people to standard ones in order to see if diets are hyper or hypo caloric...Can you help me in order to find standard norms of nutrition...
11 December 2016 1,311 0 View
Hello, I have 500 SNPS (single nucleotide polymorphisms) of cases and controls individuals, if i will use snpassoc (tool r studio) it will take too much time to insert variants, what is the best...
07 August 2016 6,241 1 View
Hello, I want to know when can we use Student's test, Pearson's correlation coefficient test and Spearman rank correlation, what can i put us matrix (numeric variables as means..sd (standard...
04 May 2016 4,542 7 View
Hello, I conserved the extracted microbial DNA from kit in -20°C (in TE BUFFER), after that i was obliged to transfer the microbial DNA to + 4°C because i can't ensure the transport from a place...
03 April 2016 6,935 3 View
Hello, For the SNP rs5219, i looked for the GMAF, i found T=0.2630/1317 but HGVS names indicated NC_000011.10:g.17388025T>C, What can i do in this case, there are some contradictions.? Thanks
02 March 2016 8,678 1 View
Hello, I need the definitions and utilities of each biostatistics tests quoted and the tutorial (tool r/matrix models) in order to achieve that. I need also the definitions and utilities of each...
10 November 2015 8,540 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View