Why they consider that Wilcoxon test and U Mann Whitney test are the same Can we use this test to examine 2 numeric variables 4 independent groups?

Hello , I'm asking you why they consider that Wilcoxon test and U Mann Whitney test are the same ? Can we use this test to examine 2 numeric variables for independent groups ? Thank you

08 September 2018 8,550 3 View

What is the best tool I can use to search for protein domains ?

Hello, I am conducting a research on the key domains of a different protein sequences. Can you give me the link of the best software that I can use to do this task ?

11 December 2017 5,686 3 View

QPCR standard curves using bacterial DNA: cloned and not cloned ?

04 May 2017 1,064 2 View

On which criterion is based the choice of the hypervariable region of bacterial rRNA16S to do NGS?

11 December 2016 1,408 1 View

Where can i find the standard norm of human nutrition book or guide ?

11 December 2016 1,164 0 View

What is the best test of association study of variants linked to controls and patients ?

Hello, I have 500 SNPS (single nucleotide polymorphisms) of cases and controls individuals, if i will use snpassoc (tool r studio) it will take too much time to insert variants, what is the best...

07 August 2016 6,080 1 View

When can we use Student's T test and Pearson test ?

Hello, I want to know when can we use Student's test, Pearson's correlation coefficient test and Spearman rank correlation, what can i put us matrix (numeric variables as means..sd (standard...

04 May 2016 4,400 7 View

Best methods of conservation of microbial DNA ?

03 April 2016 6,758 3 View

GMAF (Global Minor Allele Frequency) How identify the GMAF ?

Hello, For the SNP rs5219, i looked for the GMAF, i found T=0.2630/1317 but HGVS names indicated NC_000011.10:g.17388025T>C, What can i do in this case, there are some contradictions.? Thanks

02 March 2016 8,492 1 View

Wilcoxon. Rank Sum test/Jaccard index/Chao index/Constrained PCA/ Manhattan plot ??

10 November 2015 8,400 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View