I know from ATCC/JCRB depository saying that these cells are different based on their source and the presence of HBV/HCV. What kind of other consideration when I'm looking into working with more than one cell lines for my experiment?
It is very important to known the differences on p53 expresion. In this way, HepG2 cells carry wild-type p53, whereas Hep3B and Huh7 cells have null and point mutations at p53 codon 220 respectively.
Dear Ain,
While working with several human HCC cell lines, it was found that HepG2, PLC/PRF/5, and HuH7 showed cell proliferation inhibition by IFN-α, and PLC/PRF/5 and HuH7 had statistically significant differences in the growth inhibitory effect by IFN-α. The cell line PLC/PRF/5, which showed the most abundant expression of IFN receptors, showed the most growth inhibition and rapid inhibition of MAPK signaling and anti-proliferation effect via JAK/STAT. Although we did not examine the affected signaling cascades in all cell lines, IFN-α may show an inhibitory effect on cell proliferation through inhibition of MAPK signaling.
Best wishes,
Ilya
Another characteristics of HCC cell lines is the tumorigenic effects in vivo xenograft model. For example, HepG2 is non-tumorigenic (1 x 106) in immunosuppressed mice, but yes in semisolid medium. However, we demonstrated tumorigenic effect a high density (10 x 106) in the subcutaneous injection. Furthermore, every line showed different level of diverse proteins (Castro-Sánchez L, 2013).
It is very important to known the differences on p53 expresion. In this way, HepG2 cells carry wild-type p53, whereas Hep3B and Huh7 cells have null and point mutations at p53 codon 220 respectively.
From HBV side of view, PLC/PRF5 habours natural HBs (full-length) integration and moderate HBsAg secretion, whereas HepG2 and Huh7 cells are HBV-free. Huh7, however, compared to HepG2 cells, supports higher integration efficiency of exogenous HBV genomes, probably due to its unstable genomic feature.
As Jose wrote, p53 expression is a very important factor in research with these cell lines as it determines a lot of characteristics with regards to cell proliferation and tumor behavior (if doing xenografts). If you're transfecting the cells, then they're all really good, with efficiencies approaching 90% (see https://altogen.com/product/hepg2-transfection-reagent-hepatocellular-carcinoma/). Xenografts are likewise going to be fine, and our studies have lasted less than a month with the cell lines.
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