What are the best vectors to express sgrnas + GFP and excluding Cas9?

02 February 2016 1 2K Report

I have stable cas9 expressing mammalian cells and predesigned crisprna+tracrna (sgrna) for my GOI. However, I would like to clone sgrna into a GFP/RFP plasmid so that I can sort out these cells after transfection. By this way I know that the transfected cells have sgrna in them. Any suggestions on how to clone any sgrna into a GFP/RFP plasmid or commercial sources please.

Christiane Kuschal

You may find your answer here:

https://www.addgene.org/crispr/empty-grna-vectors/

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