What are the best vectors to express sgrnas + GFP and excluding Cas9?

02 February 2016 1 2K Report

I have stable cas9 expressing mammalian cells and predesigned crisprna+tracrna (sgrna) for my GOI. However, I would like to clone sgrna into a GFP/RFP plasmid so that I can sort out these cells after transfection. By this way I know that the transfected cells have sgrna in them. Any suggestions on how to clone any sgrna into a GFP/RFP plasmid or commercial sources please.

Badges
Science method

More Sureshbabu Angara's questions See All

How to determine which bacterial virulence factor in relevance to human sepsis or infection regulate my target protein of interest?

How to determine which bacterial virulence factor (bacterial toxins or cell wall components) in relevance to human sepsis or bacterial infection will interact or regulate my target protein of...

02 March 2016 5,923 1 View

Are there any bioinformatics tools or softwares that can locate/identify the site of binding domains between two protein sequences?

I would like to identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.

02 March 2014 9,043 0 View

Are there any bioinformatics tools or softwares that can locate/identify the site of binding domain between two protein sequences?

I would like to locate/identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.

02 March 2014 5,957 4 View

Can anyone tell me how to identify WXXL motif containing all human proteins from databases?

Can anyone suggest how to get a list (identify/filter) of all human mitochondrial proteins containing WXXL motif in their sequence from human mitochondrial protein databases?

02 March 2014 4,779 4 View

Can someone suggest inhibitors to block double strand breaks or activators that facilitate double stranded break repair process?

I noticed upregulated DNA damage (both phospho-Chk1 and phospho-Histone H2A.X) in my systems. This is followed by cycle cycle arrest and decreased CDK1/cyclin B expression. The objective is to...

04 May 2013 661 0 View

How to separate lipid contaminants during protein extraction of animal tissues?

My target protein is low molecular weight membrane protein (LC3 II). It comes out good in cell systems but not in animal lung tissues (white buffy layer on the top of the protein supernatant,...

02 March 2012 1,789 13 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View