What are the best vectors to express sgrnas + GFP and excluding Cas9?

I have stable cas9 expressing mammalian cells and predesigned crisprna+tracrna (sgrna) for my GOI. However, I would like to clone sgrna into a GFP/RFP plasmid so that I can sort out these cells...

01 February 2016 1,859 1 View

Are there any bioinformatics tools or softwares that can locate/identify the site of binding domains between two protein sequences?

I would like to identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.

02 March 2014 9,043 0 View

Are there any bioinformatics tools or softwares that can locate/identify the site of binding domain between two protein sequences?

I would like to locate/identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.

02 March 2014 5,957 4 View

Can anyone tell me how to identify WXXL motif containing all human proteins from databases?

Can anyone suggest how to get a list (identify/filter) of all human mitochondrial proteins containing WXXL motif in their sequence from human mitochondrial protein databases?

02 March 2014 4,779 4 View

Can someone suggest inhibitors to block double strand breaks or activators that facilitate double stranded break repair process?

I noticed upregulated DNA damage (both phospho-Chk1 and phospho-Histone H2A.X) in my systems. This is followed by cycle cycle arrest and decreased CDK1/cyclin B expression. The objective is to...

04 May 2013 661 0 View

How to separate lipid contaminants during protein extraction of animal tissues?

My target protein is low molecular weight membrane protein (LC3 II). It comes out good in cell systems but not in animal lung tissues (white buffy layer on the top of the protein supernatant,...

02 March 2012 1,789 13 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View